fitc conjugated maa i  (Vector Laboratories)

Journal: Journal of Virology

Article Title: Modulation of cytokeratin and cytokine/chemokine expression following influenza virus infection of differentiated human tonsillar epithelial cells

doi: 10.1128/jvi.01460-24

Figure Lengend Snippet: Distribution of CK and lectin staining in HTECs as determined by flow cytometry. ( A ) HTECs were fixed and permeabilized, then stained with monoclonal antibodies specific for CK5, CK14, CK8/18, and CK19 for flow cytometric analysis. Percentages give fraction positive for the indicated CK when gated against matched isotype controls. ( B ) HTECs were stained with MAA I-FITC lectin for avian-adapted α2,3-linked SA receptors or with SNA-BV786 lectin for mammalian-adapted α2,6-linked SA receptors. (B.a) MAA I lectin staining was nearly universal in more than five replicate experiments. (B.b) SNA lectin staining was more limited over the same experiments. (B.c) Dual staining showed that coincident expression of avian with mammalian-adapted SA in HTECs was robust. Gating is based on unstained and fluorescence-minus-one controls as shown in . Inset statistics give means ± SEM of the indicated populations from three replicate experiments.

Article Snippet: Uninfected HTECs were first stained with biotinylated SNA lectin SA and MAA I FITC-conjugated lectin (Vector Laboratories).

Techniques: Staining, Flow Cytometry, Expressing, Fluorescence

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Rapid Differentiation of Monocytes into Type I IFN-Producing Myeloid Dendritic Cells as an Antiviral Strategy against Influenza Virus Infection

doi: 10.4049/jimmunol.1200168

Figure Lengend Snippet: Influenza virus induces rapid differentiation of monocytes into mDCs. (A) Human PBMCs were cultured with Poly(I:C) (10 μg/ml), LPS (1 μg/ml), R848 (5 μg/ml), CpGA (10 μg/ml), or A/Bris/59/07 at an MOI of 1 for 16 h. Cells were then analyzed by flow cytometry to assess the frequencies of various cell types. Total DC populations were defined by a lack of lineage marker expression (CD3−CD14−CD19−CD56−) and expression of HLA-DR. (B) The percentages of CD11c+CD123low/−HLA-DR+Lin− mDCs and CD11c−CD123+HLA-DR+Lin− pDCs in PBMCs cultured with TLR ligands or A/Bris/59/07, as assessed by flow cytometry. **p < 0.01, versus mock-treated group. (C) The percentages of CD14+HLA-DR+ monocytes and DCs in PBMCs cultured with LPS or A/Bris/59/07, as assessed by flow cytometry. (D) Monocytes were isolated from fresh PBMCs by FACS sorting and cultured with A/Bris/59/07 or A/Brisbane/10/2007 at an MOI = 1. The percentages of monocytes and mDCs were analyzed by flow cytometry. (E) Expression of α-2,6–linked sialic acids was detected by lectin SNA, and expression of α-2,3–linked sialic acids was detected by MAA I on monocytes. (F) Expression of NS1 by CD11c+ mDCs as detected by intracellular flow staining. Data are representative of three independent experiments. *p < 0.05 versus mock-treated group.

Article Snippet: For detection of the expression of α−2,6–linked sialic acids and α-2,3–linked sialic acids, lectin Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA I) (both FITC conjugated) (EY Laboratories) were used.

Techniques: Virus, Cell Culture, Flow Cytometry, Marker, Expressing, Isolation, Staining